Third-degree burns are also an indication for BRT with MEBT/MEBO. 

As the epidermis and dermis of third-degree burn wound are totally destroyed, it is quite difficult to cure third-degree burns. 

The conventional medical science for burns is convinced that third-degree wounds cannot heal spontaneously, and the only solution is to use surgical skin grafting to close them. 

The clinical study and administration of BRT with MEBT/MEBO for curing third-degree burns wounds will be described in detail thereinafter. 

The following is just a brief description of the principle and method of this treatment.

Principle. 

Decompression of the deep tissues 

• relieve any pressure caused by the necrotic layer is of critical importance; 
• protect the necrotic full-thickness skin;
• promote stem cells containing the skin information in subcutaneous tissue to regenerate and differentiate to form a skin island; 
• culture the newly regenerated skin island while liquefying and discharging the necrotic dermis; 
• promote the skin island to spread and cover subcutaneous tissue to form new skin; 
• and help third-degree burns wounds heal spontaneously. 

For third-degree wounds injured down to the muscle layer, excise most of the necrotic tissue by surgical operation, liquefy and discharge the rest of the necrotic layer with BRT with MEBT/MEBO, upon which culture granulation tissue, then plant skin cells till the wound closes and heals.

For wounds with bone exposed, clean away the exposed outer soft tissue, drill holes on the bone surface with a bone drill at intervals of 0.5–1 cm, deep into medullary cavity of bone until bleeding. Apply MEBO to cover the wounds, and culture and support the growth of granulation tissue from the holes. When the granulation tissue spreads to cover the bone surface, skin grafting can be performed to close the wound, or the wound heals by migration of epithelial cells from the wound edges.

The necrotic tissue of third-degree burns should be decompressed by cutting both horizontally and vertically at a 1 mm tooth distance and depth with a specially designed method: ‘plough saw blade’. Then apply MEBO for protection and clean away the exfoliated or liquefied tissue.

1. III degree burn with area of 85%TBSA.The pathological section at right side shows full-thickness skin necrosis.

2. Apply cultivating and tension relieving method on III degree wounds on chest. Apply MEBT/MEBO to treat.

3. Clean liquefied necrotic tissues in time and treat wounds complying with the principle of treating deep IIdegree burns.

4. On 20th day postburn, burned necrotic tissues are liquefied and discharged. A layer of membrane forms on wounds. The pathological section shows that wounds were repaired by regenerative skin tissues.

5. Under the protection of MEBO and membrane, regenerative skin tissues form dense skin islands.

6. Pathological section: stem cells regenerate and repair skin in situ.

7. 'Skin islands' gradually extend to normal skin tissues.

8. On 49th day postburn, the pathological section of healed skin shows structure of normal skin.

9 . On 7 2nd day postburn, whole layers of skin regenerate, repair and heal.